Endonuclease G Protein (ENDOG) (AA 1-294) (His tag,GST tag)
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- Target See all Endonuclease G (ENDOG) Proteins
- Endonuclease G (ENDOG)
- Biological Activity
- Active
- Protein Characteristics
- AA 1-294
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Origin
- Rat
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Endonuclease G protein is labelled with His tag,GST tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His and GST Tag
- Purity
- > 90 %
- Biological Activity Comment
- ENDOG (Endonuclease G) is a mitochondrial enzyme that cleaves DNA at double-stranded (DG)n. (DC)n and at single-stranded (DC)n tracts. This protein also has ribonuclease (RNase) and RNase H activities. It has been reported that EndoG forms complexes with some other proteins, including HSP70 (Heat shock 70 kDa protein). Besides, mouse HSPA1A (Heat shock 70 kDa protein 1A) shares similarities with rat HSP1A1 in amino acid sequence with the identity of 98.44%. Thus, a binding ELISA assay was conducted to detect the association of recombinant rat ENDOG with recombinant mouse HSPA1A. Briefly, ENDOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP1A1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ENDOG with HSP1A1 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of ENDOG with HSP1A1
- Top Product
- Discover our top product ENDOG Protein
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Endonuclease G (ENDOG) (AA 23-212) protein (His-IF2DI Tag)
ENDOG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Endonuclease G (ENDOG) (AA 50-297) protein (GST tag)ENDOG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endonuclease G (ENDOG) (AA 51-140) protein (His tag,Strep II tag)ENDOG Origin: Human Host: Escherichia coli (E. coli) Recombinant ~95 % WB, ELISA, MS
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- Application Notes
- Isoelectric Point: 9.5
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Endonuclease G (ENDOG)
- Alternative Name
- Endonuclease G, Mitochondrial (ENDOG) (ENDOG Products)
- Synonyms
- CG8862 Protein, Dmel\\CG8862 Protein, Tb08.10J17.140 Protein, Tb08.10J17.90 Protein, TBC1D13 Protein, endog Protein, ENDOG Protein, wu:fb79c08 Protein, zgc:110020 Protein, Endonuclease G Protein, endonuclease G Protein, putative endonuclease G Protein, endonuclease G, mitochondrial Protein, endonuclease G L homeolog Protein, EndoG Protein, Tc00.1047053506867.10 Protein, Tb927.8.4040 Protein, Tb927.8.4090 Protein, LBRM_10_0750 Protein, LMJF_10_0610 Protein, NAEGRDRAFT_78507 Protein, ENDOG Protein, endog Protein, eNDOG Protein, LOC100529218 Protein, Endog Protein, endog.L Protein
- Background
- Alternative Names: ENDO-G
- Molecular Weight
- 62kDa
- UniProt
- Q3V5X8
- Pathways
- Apoptosis
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