WNT7A Protein

Catalog No. ABIN2469224

Product Details

Target: WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Sequence: LGASIICNKI PGLAPRQRAI CQSRPDAIIV IGEGSQMGLD ECQFQFRNGR WNCSALGERT VFGKELKVGS REAAFTYAII AAGVAHAITA ACTQGNLSDC GCDKEKQGQY HRDEGWKWGG CSADIRYGIG FAKVFVDARE IKQNARTLMN LHNNEAGRKI LEENMKLECK CHGVSGSCTT KTCWTTLPQF RELGYVLKDK YNEAVHVEPV RASRNKRPTF LKIKKPLSYR KPMDTDLVYI EKSPNYCEED PVTGSVGTQG RACNKTAPQA SGCDLMCCGR GYNTHQYARV WQCNCKFHWC CYVKCNTCSE RTEMYTCK

Characteristics: Biological activity was determined by its ability to inhibit Wnt3a induced alkaline phosphatase production in MC3T3-E1 cells. The expected ED50 for this effect is 40-60 ng/mL.

Purity: < 80 % by SDS-PAGE gel and HPLC analyses.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing WNT-7A recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted WNT-7A stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: WNT7A (Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A))

Alternative Name: WNT-7A (WNT7A Products)

Synonyms: wnt7a Protein, AI849442 Protein, Wnt-7a Protein, px Protein, tw Protein, Xwnt-7a Protein, wnt-7a Protein, wnt7a-A Protein, Wnt family member 7A Protein, wingless-type MMTV integration site family, member 7Aa Protein, wingless-type MMTV integration site family, member 7A Protein, wingless-type MMTV integration site family member 7A S homeolog Protein, WNT7A Protein, wnt7aa Protein, Wnt7a Protein, wnt7a.S Protein

Background: Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of ?-Catenein degradation. Intracellular accumulation of beta-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/?-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions.

Gene ID: 7476

NCBI Accession: NP_004616

OMIM: 17505191

UniProt: O00755

Pathways: WNT Signaling, Stem Cell Maintenance, Asymmetric Protein Localization