CCL5 Protein (AA 24-91)

Catalog No. ABIN2666976

Product Details

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Protein Type: Recombinant

Protein Characteristics: AA 24-91

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Flow Cytometry (FACS)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin Level:

Less than 0.01ng/μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Alternative Name: CCL5 (CCL5 Products)

Synonyms: Rantes Protein, Scya5 Protein, D17S136E Protein, RANTES Protein, SCYA5 Protein, SIS-delta Protein, SISd Protein, TCP228 Protein, eoCP Protein, MuRantes Protein, C-C motif chemokine 5-like Protein, C-C motif chemokine ligand 5 Protein, chemokine (C-C motif) ligand 5 Protein, LOC100230935 Protein, CCL5 Protein, Ccl5 Protein

Background: CCL5 (RANTES), also known as CCL5, is a 7.8 kD member of the CC family of chemokines, which is expressed in T cells and monocytes/macrophages. RANTES is strongly chemotactic for monocytes/macrophages, memory T cells, eosinophils, and induces histamine release from basophils. In a solution, RANTES can form high molecular weight aggregates that exhibit dissimilar biological activities to its dimeric form. The aggregates cause T cell and neutrophil activation while promoting HIV infection, whereas the dimeric form exhibits RANTES-induced chemotaxis and inhibits HIV infection. As T cells become activated, RANTES secretion appears to decrease.

Molecular Weight: The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.851 kDa. The DTT-reduced protein migrates at approximately 7 kDa and the non-reduced protein migrates at approximately 9 kDa by SDS-PAGE. The N-terminal amino acid is

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration