Quick Overview for p53 Protein (full length) (ABIN2669408)
Target
See all p53 (TP53) ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- full length
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Characteristics
- Full-length recombinant p53 protein was expressed in a baculovirus system with a polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. Active Motif also offers Recombinant p53 protein (Catalog No. 31465) that was expressed in E. coli.
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Purification
- Purified by an affinity column in combination with FPLC chromatography.
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Tumor Protein P53 (TP53) (AA 1-393) protein (His tag)
custom-made TP53 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Protein P53 (TP53) (AA 1-393) protein (GST tag)TP53 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Protein P53 (TP53) (Active) protein (His tag)TP53 Origin: Human Host: Escherichia coli (E. coli) Recombinant STD, PI, in vitro Active
Tumor Protein P53 (TP53) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TP53 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Tumor Protein P53 (TP53) (AA 1-391) protein (His tag)TP53 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Tumor Protein P53 (TP53) protein (His-SUMO Tag)TP53 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90% as determined by SDS-PAGE. ELISA
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Application Notes
- Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 - 0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
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Restrictions
- For Research Use only
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Concentration
- 0.5 μg/μL
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Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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Storage
- -20 °C/-80 °C
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Storage Comment
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- p53
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Background
- P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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