p53 Protein (His tag)

Catalog No. ABIN2669713

This Recombinant p53 protein is produced in Escherichia coli (E. coli).

Quick Overview for p53 Protein (His tag) (ABIN2669713)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Standard (STD), Protein Interaction (PI), In vitro Assay (in vitro)

Product Details

Purification tag / Conjugate: This p53 protein is labelled with His tag.

Characteristics: p53 is a tumor suppressor protein functioning as a sequence-specific transcription factor important for cell cycle arrest, apoptosis, and DNA-damage response. Recombinant p53, transcript variant 1, was expressed in E. coli from human cDNA (accession number NM_000546.5) with an N-terminal polyhistidine tag. Active Motif also offers Recombinant p53 protein (Catalog No. 31103) that was expressed in Baculovirus.

Application Details

Application Notes: Recombinant p53 is suitable for use in Active Motif's TransAM® assay as a protein standard, in vitro transcription assays, protein-protein interactions and protein-DNA interaction studies. A recommended starting point for in vitro transcription assays is 20-100 ng per reaction. The molecular weight of the protein is 53 kDa.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect