NR1H4 Protein (His tag)

Catalog No. ABIN2669415

Product Details

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NR1H4 protein is labelled with His tag.

Application: Protein Interaction (PI)

Characteristics: Recombinant FXR is isolated from an E. coli strain that carries the coding sequence of the human FXR under the control of a T7 promoter (accession number U68233). The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity.

Purity: The purified recombinant protein has an amino terminal polyhistidine tag and is greater than 90 % homogeneous and contains no detectable protease, DNase and RNase activity.

Application Details

Application Notes: Recombinant FXR is suitable for DNA and protein-protein interaction assays. 20 ng is sufficient for gelshift assays and 100 ng is sufficient for protein-protein interaction studies. The molecular weight of the protein is ~55 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Restrictions: For Research Use only

Handling

Concentration: 0.1 μg/μL

Target Details

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Alternative Name: FXR (NR1H4 Products)

Synonyms: zgc:110190 Protein, zgc:92742 Protein, NR1H4 Protein, BAR Protein, FXR Protein, HRR-1 Protein, HRR1 Protein, RIP14 Protein, AI957360 Protein, Fxr Protein, Rxrip14 Protein, nuclear receptor subfamily 1, group H, member 5 S homeolog Protein, nuclear receptor subfamily 1, group H, member 4 Protein, nuclear receptor subfamily 1 group H member 4 Protein, nr1h5.S Protein, nr1h4 Protein, NR1H4 Protein, Nr1h4 Protein

Background: The farnesoid X receptor (FXR) or nuclear receptor subfamily 1, group H, member 4 (NR1H4) is a nuclear receptor that, when activated, translocates to the nucleus where it interacts with certain proteins, including RXR and PPARGC1A, forming heterodimers that bind to hormone response elements on DNA and modulate gene expression. One of the primary functions of FXR is to suppress bile acid synthesis when levels are high by inhibiting the transcription of cholesterol 7 alpha-hydroxylase (CYP7A1) gene that expresses CYP7A1, the rate-limiting enzyme in bile acid synthesis from cholesterol.

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process