CD31 Protein

Catalog No. ABIN2713636

Product Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human CD31 / PECAM1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5ug/mL.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: Cd31,pecam1 (PECAM1 Products)

Synonyms: CD31 Protein, CD31/EndoCAM Protein, GPIIA' Protein, PECA1 Protein, PECAM-1 Protein, endoCAM Protein, C85791 Protein, Cd31 Protein, Pecam Protein, platelet/endothelial cell adhesion molecule 1 Protein, platelet and endothelial cell adhesion molecule 1 Protein, pecam1 Protein, PECAM1 Protein, Pecam1 Protein

Background: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

Molecular Weight: 64 kDa

NCBI Accession: NP_000433

Pathways: Regulation of Actin Filament Polymerization