Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- Myc-DYKDDDDK Tag
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human ACADM (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-100) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-421) protein (GST tag)Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Alternative Name
- Acadm
- Synonyms
- ACAD1 Protein, MCAD Protein, MCADH Protein, AU018656 Protein, acyl-CoA dehydrogenase medium chain Protein, acyl-Coenzyme A dehydrogenase, medium chain Protein, acyl-CoA dehydrogenase, C-4 to C-12 straight chain Protein, ACADM Protein, Acadm Protein
- Background
- This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 43.6 kDa
- NCBI Accession
- NP_000007
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