Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial protein (His tag)
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human ACADM/MCAD Protein (His Tag)
- Sequence
- Lys26-Asn421
- Characteristics
- Recombinant Human Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial is produced by our E.coli expression system and the target gene encoding Lys26-Asn421 is expressed with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-100) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-421) protein (GST tag)Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 0.1M NaCl, 20 % Glycerol, pH 8.5.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Alternative Name
- ACADM/MCAD
- Synonyms
- ACAD1 Protein, MCAD Protein, MCADH Protein, AU018656 Protein, acyl-CoA dehydrogenase medium chain Protein, acyl-Coenzyme A dehydrogenase, medium chain Protein, acyl-CoA dehydrogenase, C-4 to C-12 straight chain Protein, ACADM Protein, Acadm Protein
- Background
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Background: Medium-Chain Specific Acyl-CoA Dehydrogenase (ACADM) is a mitochondrial fatty acid beta-oxidation that belongs to the acyl-CoA dehydrogenase family. ACADM is a homotetramer enzyme that catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. ACADM is specific for acyl chain lengths of 4 to 16. It is essential for converting these particular fatty acids to energy, especially during fasting periods. Defects in ACADM cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death.
Synonym: Medium-Chain Specific Acyl-CoA Dehydrogenase Mitochondrial, MCAD, ACADM
- Molecular Weight
- 45.9 kDa
- UniProt
- P11310
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