DGKE Protein (Myc-DYKDDDDK Tag)
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- Target See all DGKE Proteins
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DGKE protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DAG kinase epsilon protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DGKE Protein
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Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-177) protein (GST tag)
DGKE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 141-240) protein (GST tag)DGKE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-567) protein (Strep Tag)Crystallography grade DGKE Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-564) protein (Strep Tag)Crystallography grade DGKE Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 243-502) (Active) protein (His tag)DGKE Origin: Human Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
- Alternative Name
- Dag Kinase epsilon (DGKE Products)
- Synonyms
- MGC81643 Protein, DGKE Protein, C87606 Protein, DAGK6 Protein, DGK Protein, DAGK5 Protein, NPHS7 Protein, diacylglycerol kinase epsilon S homeolog Protein, diacylglycerol kinase epsilon Protein, diacylglycerol kinase, epsilon Protein, dgke.S Protein, DGKE Protein, dgke Protein, LOC5575244 Protein, Dgke Protein
- Background
- Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
- Molecular Weight
- 63.7 kDa
- NCBI Accession
- NP_003638
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