DGKE Protein (AA 243-502) (His tag)

Catalog No. ABIN6239838

Product Details

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Biological Activity: Active

Protein Characteristics: AA 243-502

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DGKE protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: DGKe (Diacylglycerol kinase epsilon) is an enzyme that mainly involved in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha) has been proven to be an interactor of DGKe through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human DGKe and recombinant human PDHA. Briefly, DGKe were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL DGKe were then transferred to PDHA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-DGKe pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of DGKe and PDHA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of DGKe with PDHA.

Application Details

Application Notes: Isoelectric Point: 7.7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Alternative Name: Diacylglycerol Kinase Epsilon (DGKe) (DGKE Products)

Synonyms: MGC81643 Protein, DGKE Protein, C87606 Protein, DAGK6 Protein, DGK Protein, DAGK5 Protein, NPHS7 Protein, diacylglycerol kinase epsilon S homeolog Protein, diacylglycerol kinase epsilon Protein, diacylglycerol kinase, epsilon Protein, dgke.S Protein, DGKE Protein, dgke Protein, LOC5575244 Protein, Dgke Protein

Background: Alternative Names: DAGK5, DGK, Diglyceride kinase epsilon

Molecular Weight: 33kDa

UniProt: P52429