DPM2 Protein (Myc-DYKDDDDK Tag)
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- Target See all DPM2 Proteins
- DPM2 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 2, Regulatory Subunit (DPM2))
- Protein Type
- Recombinant
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DPM2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DPM2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- DPM2 (Dolichyl-Phosphate Mannosyltransferase Polypeptide 2, Regulatory Subunit (DPM2))
- Alternative Name
- Dpm2 (DPM2 Products)
- Synonyms
- dpm2b Protein, dpm2a Protein, AW557993 Protein, R75484 Protein, CDG1U Protein, wu:fa99c01 Protein, zgc:112514 Protein, dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit S homeolog Protein, dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit L homeolog Protein, dolichol-phosphate (beta-D) mannosyltransferase 2 Protein, dolichyl-phosphate mannosyltransferase subunit 2, regulatory Protein, dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit Protein, dpm2.S Protein, dpm2.L Protein, Dpm2 Protein, DPM2 Protein, dpm2 Protein
- Background
- Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1.
- Molecular Weight
- 9.1 kDa
- NCBI Accession
- NP_003854
- Pathways
- Inositol Metabolic Process
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