EPO Protein

Catalog No. ABIN2720509

This Recombinant EPO protein is expressed in CHO Cells.

Quick Overview for EPO Protein (ABIN2720509)

Target: EPO (Erythropoietin (EPO))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Application: Functional Studies (Func), Protein Interaction (PI), Standard (STD), Antibody Production (AbP)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Erythropoietin / EPO protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2 x 10^6 units/mg, determined by the dose dependent stimulation of TF-1 cells proliferation.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: EPO (Erythropoietin (EPO))

Alternative Name: Erythropoietin,epo

Target Type: Hormone

Molecular Weight: 18.6 kDa

NCBI Accession: NP_031968

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity