EPO Protein
-
- Target See all EPO Proteins
- EPO (Erythropoietin (EPO))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Mouse
-
Source
- CHO Cells
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
-
- Recombinant human Erythropoietin / EPO protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2 x 10^6 units/mg, determined by the dose dependent stimulation of TF-1 cells proliferation.
- Top Product
- Discover our top product EPO Protein
-
Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Erythropoietin (EPO) (Active) protein (His tag)EPO Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
-
- Concentration
- > 50 μg/mL
- Buffer
- 1 x PBS
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- EPO (Erythropoietin (EPO))
- Alternative Name
- Erythropoietin,epo (EPO Products)
- Synonyms
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- Target Type
- Hormone
- Molecular Weight
- 18.6 kDa
- NCBI Accession
- NP_031968
- Pathways
- JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
-
