GATA4 Protein (Myc-DYKDDDDK Tag)
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- Target See all GATA4 Proteins
- GATA4 (GATA Binding Protein 4 (GATA4))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GATA4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human GATA4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- GATA4 Activity Verified in a DNA-binding Assay: Comparison of GATA4 binding to consensus and mutant oligos shows sensitivity of protein binding to the DNA sequence used.
- Top Product
- Discover our top product GATA4 Protein
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GATA Binding Protein 4 (GATA4) (AA 301-442) protein (His-IF2DI Tag)
GATA4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
GATA Binding Protein 4 (GATA4) (AA 343-442) protein (GST tag)GATA4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
GATA Binding Protein 4 (GATA4) (AA 1-442) protein (Strep Tag)Crystallography grade GATA4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
GATA Binding Protein 4 (GATA4) (AA 1-441) protein (Strep Tag)Crystallography grade GATA4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
GATA Binding Protein 4 (GATA4) proteinGATA4 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GATA4 (GATA Binding Protein 4 (GATA4))
- Alternative Name
- Gata4 (GATA4 Products)
- Synonyms
- gata-4 Protein, xgata4 Protein, xGATA-4 Protein, MGC81427 Protein, GATA4 Protein, GATA-4 Protein, Gata4 Protein, gata4 Protein, ASD2 Protein, VSD1 Protein, Gata-4 Protein, gata Protein, GATA binding protein 4 Protein, GATA binding protein 4 L homeolog Protein, glutamyl-tRNA(Gln) amidotransferase subunit A Protein, gata4 transcription factor Protein, GATA binding protein 4 S homeolog Protein, GATA4 Protein, gata4.L Protein, gata4 Protein, gatA4 Protein, Gata4 Protein, gata4.S Protein
- Background
- This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 44.4 kDa
- NCBI Accession
- NP_002043
- Pathways
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
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