GBA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721979

Product Details

Target: GBA (Glucosidase, Beta, Acid (GBA))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GBA protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Glucosylceramidase (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: The enzymatic activity of (GBA) was measured by its ability to hydrolyze a fluorescent substrate 4-methylumbelliferyl-ß-D-glucopyranoside. The specific activity is > 70,000 pmol/hour/μg, as measured under the following conditions: 27 ng of GBA was incubated with 10 mM 4-methylumbelliferyl- ß-D-glucopyranoside in the following buffer at 37°C for 40 min: 150 mM citrate-phosphate buffer, pH 5.4, 0.25% (w/w) sodium taurocholate, 0.25% (w/w) Triton X-100, and 1% bovine serum albumin. The reaction was terminated by adding 0.5 volume of 1M glycine buffer, pH 12.5. The hydrolyzed product of reaction, 4-methylumbelliferone (4-MU), was measured using a FlexStation 3 microplate reader (Ex365/Em445). Specific activity of GBA was calculated based on a standard curve of known concentration of 4-MU.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GBA (Glucosidase, Beta, Acid (GBA))

Alternative Name: Glucosylceramidase (GBA Products)

Synonyms: GBA1 Protein, GCB Protein, GLUC Protein, BETA-GLUCOSIDASE Protein, T12J13.8 Protein, T12J13_8 Protein, beta glucosidase 25 Protein, PSPTO3318 Protein, PSPTO4290 Protein, GC Protein, GCase Protein, betaGC Protein, glucosylceramidase beta Protein, beta glucosidase 25 Protein, beta-glucosidase Protein, Beta-glucosidase Protein, glucosidase, beta, acid Protein, glucosylceramidase Protein, bglX-2 Protein, GBA Protein, BGLU25 Protein, PSPTO_3318 Protein, bglX Protein, bglA4 Protein, Gba Protein, LOC100399524 Protein, bglA.2 Protein, bglX-2 Protein

Background: This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.

Molecular Weight: 55.5 kDa

NCBI Accession: NP_000148

Pathways: Cellular Glucan Metabolic Process