IL-34 Protein (Transcript Variant 1) (His tag)
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- Target See all IL-34 (IL34) Proteins
- IL-34 (IL34) (Interleukin 34 (IL34))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-34 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Interleukin-34 / IL34 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Measured by its ability to induce secretion of MCP-1 from PBMCs.
- Top Product
- Discover our top product IL34 Protein
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Interleukin 34 (IL34) (AA 21-242) protein (His tag)
Crystallography grade IL34 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 34 (IL34) (AA 21-235) protein (His tag)Crystallography grade IL34 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 34 (IL34) proteinIL34 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Interleukin 34 (IL34) protein (Myc-DYKDDDDK Tag)IL34 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL-34 (IL34) (Interleukin 34 (IL34))
- Alternative Name
- Interleukin-34,il34 (IL34 Products)
- Synonyms
- C16orf77 Protein, IL-34 Protein, 2010004A03Rik Protein, AI593503 Protein, C18H16ORF77 Protein, interleukin 34 Protein, IL34 Protein, Il34 Protein
- Background
- Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008].
- Molecular Weight
- 52.5 kDa
- NCBI Accession
- NP_689669
- Pathways
- RTK Signaling
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