IL-34 Protein (Transcript Variant 1) (His tag)
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- Target See all IL-34 (IL34) Proteins
- IL-34 (IL34) (Interleukin 34 (IL34))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Mouse
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Source
- CHO Cells
- Purification tag / Conjugate
- This IL-34 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Interleukin-34 / IL34 (transcript variant 1) protein expressed in CHO cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 20 - 30 ng/ml, corresponding to a specific activity of 3.3 - 5 x 10^4 units/mg, determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
- Top Product
- Discover our top product IL34 Protein
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Interleukin 34 (IL34) (AA 21-242) protein (His tag)
Crystallography grade IL34 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 34 (IL34) (AA 21-235) protein (His tag)Crystallography grade IL34 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 34 (IL34) proteinIL34 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Interleukin 34 (IL34) protein (Myc-DYKDDDDK Tag)IL34 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- IL-34 (IL34) (Interleukin 34 (IL34))
- Alternative Name
- Interleukin-34,il34 (IL34 Products)
- Synonyms
- C16orf77 Protein, IL-34 Protein, 2010004A03Rik Protein, AI593503 Protein, C18H16ORF77 Protein, interleukin 34 Protein, IL34 Protein, Il34 Protein
- Molecular Weight
- 26 kDa
- NCBI Accession
- NP_001128572
- Pathways
- RTK Signaling
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