IDH3G Protein (His tag)
-
- Target See all IDH3G Proteins
- IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IDH3G protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human Isocitric dehydrogenase gamma / IDH3G (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product IDH3G Protein
-
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the N-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- IDH3G (Isocitrate Dehydrogenase 3 (NAD+) gamma (IDH3G))
- Alternative Name
- Isocitric Dehydrogenase Gamma,idh3g (IDH3G Products)
- Synonyms
- H-IDHG Protein, MGC83400 Protein, wu:fa01b02 Protein, wu:fc88f06 Protein, h-idhg Protein, IDH Protein, isocitrate dehydrogenase 3 (NAD(+)) gamma Protein, isocitrate dehydrogenase 3 (NAD+) gamma S homeolog Protein, isocitrate dehydrogenase 3 (NAD+) gamma Protein, isocitrate dehydrogenase 3 (NAD+), gamma Protein, isocitrate dehydrogenase 3 (NAD), gamma Protein, IDH3G Protein, idh3g.S Protein, idh3g Protein, Idh3g Protein
- Background
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.
- Molecular Weight
- 38.8 kDa
- NCBI Accession
- NP_004126
-