MAP2K4 Protein (Myc-DYKDDDDK Tag)
Quick Overview for MAP2K4 Protein (Myc-DYKDDDDK Tag) (ABIN2725393)
Target
See all MAP2K4 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This MAP2K4 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human MAP2K4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-144) protein (His-IF2DI Tag)
MAP2K4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) protein (His-GST)MAP2K4 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 1-399) protein (GST tag)MAP2K4 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (AA 2-397) protein (His-SUMO Tag)MAP2K4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))
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Alternative Name
- Map2k4
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Background
- This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 44.1 kDa
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NCBI Accession
- NP_003001
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Pathways
- MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
Target
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