MAP2K4 Protein (AA 2-397) (His-SUMO Tag)

Catalog No. ABIN5709899

Product Details

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Protein Type: Recombinant

Protein Characteristics: AA 2-397

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MAP2K4 protein is labelled with His-SUMO Tag.

Application: SDS-PAGE (SDS)

Sequence: AAPSPSGGGG SGGGGGTPGP IGPPASGHPA VSSMQGKRKA LKLNFANPPV KSTARFTLNP NTTGVQNPHI ERLRTHSIES SGKLKISPEQ HWDFTAEDLK DLGEIGRGAY GSVNKMVHKP SGQIMAVKRI RSTVDEKEQK QLLMDLDVVM RSSDCPYIVQ FYGALFREGD CWICMELMST SFDKFYKYVY SVLDDVIPEE ILGKITLATV KALNHLKENL KIIHRDIKPS NILLDRSGNI KLCDFGISGQ LVDSIAKTRD AGCRPYMAPE RIDPSASRQG YDVRSDVWSL GITLYELATG RFPYPKWNSV FDQLTQVVKG DPPQLSNSEE REFSPSFINF VNLCLTKDES KRPKYKELLK HPFILMYEER TVEVACYVCK ILDQMPATPS SPMYVD

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: MP2K4 (MAP2K4 Products)

Synonyms: JNKK Protein, JNKK1 Protein, MAPKK4 Protein, MEK4 Protein, MKK4 Protein, PRKMK4 Protein, SAPKK-1 Protein, SAPKK1 Protein, SEK1 Protein, SERK1 Protein, SKK1 Protein, Sek1 Protein, Serk1 Protein, MKK4A-l Protein, map2k4 Protein, zgc:136500 Protein, jnkk Protein, mek4 Protein, mkk4 Protein, sek1 Protein, jnkk1 Protein, serk1 Protein, xmek2 Protein, mapkk4 Protein, prkmk4 Protein, 142758_at Protein, CG9738 Protein, CT27508 Protein, D-MKK4 Protein, DMKK4 Protein, Dmel\\CG9738 Protein, JNKK2 Protein, Mpk4 Protein, SEK1/MKK4 Protein, dMKK4 Protein, dMkk4 Protein, hep Protein, ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4 Protein, ATMEK4 Protein, ATMKK4 Protein, F19C24.26 Protein, F19C24_26 Protein, MAP KINASE KINASE 4 Protein, mitogen-activated protein kinase kinase 4 Protein, MKK4B Protein, zgc:194486 Protein, mitogen-activated protein kinase kinase 4 Protein, mitogen-activated protein kinase kinase 4a Protein, MAP kinase kinase 4 Protein, mitogen activated protein kinase kinase 4 Protein, mitogen-activated protein kinase kinase 4b Protein, MAP2K4 Protein, Map2k4 Protein, map2k4a Protein, map2k4 Protein, Mkk4 Protein, MKK4 Protein, map2k4b Protein

Background: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 ses to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

Molecular Weight: 60 kDa

UniProt: P47809

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling