MID1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for MID1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2726004)
Target
See all MID1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This MID1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Midline-1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Midline 1 (MID1) (AA 1-680) protein (His tag)
custom-made MID1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Midline 1 (MID1) (AA 1-667) protein (His tag)custom-made MID1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Midline 1 (MID1) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)MID1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Midline 1 (MID1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)MID1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MID1 (Midline 1 (MID1))
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Alternative Name
- Midline-1
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Background
- The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the &aposRING-B box-coiled coil&apos (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.
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Molecular Weight
- 75.1 kDa
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NCBI Accession
- NP_000372
Target
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