MRPL53 Protein (Myc-DYKDDDDK Tag)
MRPL53
Origin: Human
Host: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target See all MRPL53 products
- MRPL53 (Mitochondrial Ribosomal Protein L53 (MRPL53))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MRPL53 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Mitochondrial Ribosomal Protein L53 (MRPL53) (AA 1-112) protein (His-IF2DI Tag)
MRPL53 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MRPL53 (Mitochondrial Ribosomal Protein L53 (MRPL53))
- Abstract
- MRPL53 Products
- Synonyms
- L53MT Protein, 1110007K17Rik Protein, mitochondrial ribosomal protein L53 Protein, MRPL53 Protein, Mrpl53 Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p.
- Molecular Weight
- 11.9 kDa
- NCBI Accession
- NP_444278
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