MRPS33 Protein (His tag)
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- Target See all MRPS33 Proteins
- MRPS33 (Mitochondrial Ribosomal Protein S33 (MRPS33))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MRPS33 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MRPS33 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MRPS33 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 100 mM Arg. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MRPS33 (Mitochondrial Ribosomal Protein S33 (MRPS33))
- Alternative Name
- Mrps33 (MRPS33 Products)
- Synonyms
- CG10406 Protein, Dmel\\CG10406 Protein, MRP-S33 Protein, zgc:110295 Protein, PTD003 Protein, S33mt Protein, AI841153 Protein, CGI-139 Protein, Gdap3 Protein, mitochondrial ribosomal protein S33 Protein, mitochondrial ribosomal protein S33 L homeolog Protein, 28S ribosomal protein S33, mitochondrial Protein, mRpS33 Protein, MRPS33 Protein, mrps33 Protein, mrps33.L Protein, LOC725620 Protein, Mrps33 Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q
- Molecular Weight
- 12.6 kDa
- NCBI Accession
- NP_057155
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