NEDD4 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727061

Product Details

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NEDD4 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human NEDD4 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Alternative Name: Nedd4 (NEDD4 Products)

Synonyms: NEDD4-1 Protein, RPF1 Protein, AA959633 Protein, AL023035 Protein, AU019897 Protein, E430025J12Rik Protein, Nedd4-1 Protein, Nedd4a Protein, mKIAA0093 Protein, CG32184 Protein, CG42279 Protein, CG7555 Protein, DNedd4 Protein, Dmel\\CG42279 Protein, Dmel_CG32184 Protein, Dmel_CG7555 Protein, dNedd4 Protein, dNedd4-1 Protein, nedd4 Protein, GB16853 Protein, NEDD4 Protein, Nedd4 Protein, neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase Protein, neural precursor cell expressed, developmentally down-regulated 4 Protein, CG42279 gene product from transcript CG42279-RJ Protein, E3 ubiquitin-protein ligase Nedd-4 Protein, E3 ubiquitin-protein ligase NEDD4 Protein, NEDD4 Protein, Nedd4 Protein, LOC411723 Protein, LOC100457150 Protein, nedd4 Protein, LOC100547485 Protein

Background: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

Molecular Weight: 104 kDa

NCBI Accession: NP_006145

Pathways: Notch Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2