NEDD4-2 Protein (Transcript Variant B) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2727064

Product Details

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant B

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NEDD4-2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human NEDD4L (transcript variant b) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Alternative Name: Nedd4l (NEDD4L Products)

Synonyms: NEDD4-2 Protein, NEDD4.2 Protein, RSP5 Protein, hNEDD4-2 Protein, nedd4 Protein, nedd4-2 Protein, NEDD4 Protein, Nedd4-2 Protein, 1300012C07Rik Protein, Nedd4b Protein, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase Protein, NEDD4L Protein, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog Protein, neural precursor cell expressed, developmentally down-regulated gene 4-like Protein, NEDD4L Protein, LOC776799 Protein, nedd4l.S Protein, Nedd4l Protein

Background: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 98 kDa

NCBI Accession: NP_001138436

Pathways: Negative Regulation of Transporter Activity