PLEKHM2 Protein (Myc-DYKDDDDK Tag)
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- Target See all PLEKHM2 Proteins
- PLEKHM2 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 2 (PLEKHM2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PLEKHM2 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PLEKHM2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PLEKHM2 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PLEKHM2 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 2 (PLEKHM2))
- Alternative Name
- Plekhm2 (PLEKHM2 Products)
- Synonyms
- zgc:194971 Protein, RP11-169K16.1 Protein, SKIP Protein, 2310034J19Rik Protein, AI854247 Protein, mKIAA0842 Protein, pleckstrin homology and RUN domain containing M2 Protein, pleckstrin homology domain containing, family M (with RUN domain) member 2 Protein, PLEKHM2 Protein, plekhm2 Protein, Plekhm2 Protein
- Background
- This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction.
- Molecular Weight
- 112.6 kDa
- NCBI Accession
- NP_055979
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