PML Protein (Transcript Variant 9) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729208

This Recombinant PML protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for PML Protein (Transcript Variant 9) (Myc-DYKDDDDK Tag) (ABIN2729208)

Target: PML (Promyelocytic Leukemia (PML))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 9

Purification tag / Conjugate: This PML protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human PML / RNF71 (transcript variant 9) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Missiroli, Perrone, Gafà, Nicoli, Bonora, Morciano, Boncompagni, Marchi, Lebiedzinska-Arciszewska, Vezzani, Lanza, Kricek, Borghi, Fiorica, Ito, Wieckowski, Di Virgilio, Abelli, Pinton, Giorgi: "PML at mitochondria-associated membranes governs a trimeric complex with NLRP3 and P2X7R that modulates the tumor immune microenvironment." in: Cell death and differentiation, pp. 1-13, (2022) (PubMed).

Target Details

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: Pml,rnf71

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 90.5 kDa

NCBI Accession: NP_150242

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus