PML Protein (Transcript Variant 5) (Myc-DYKDDDDK Tag)
-
- Target See all PML Proteins
- PML (Promyelocytic Leukemia (PML))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 5
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PML protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human PML / RNF71 (transcript variant 5) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PML Protein
-
Promyelocytic Leukemia (PML) (AA 411-510) protein (GST tag)
PML Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Promyelocytic Leukemia (PML) (AA 1-882) protein (Strep Tag)Crystallography grade PML Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Promyelocytic Leukemia (PML) (AA 1-885) protein (Strep Tag)Crystallography grade PML Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Promyelocytic Leukemia (PML) (Transcript Variant 9) protein (Myc-DYKDDDDK Tag)PML Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Promyelocytic Leukemia (PML) (Transcript Variant 7) protein (Myc-DYKDDDDK Tag)PML Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- PML (Promyelocytic Leukemia (PML))
- Alternative Name
- Pml,rnf71 (PML Products)
- Synonyms
- MYL Protein, PP8675 Protein, RNF71 Protein, TRIM19 Protein, 1200009E24Rik Protein, AI661194 Protein, Trim19 Protein, RGD1562602 Protein, PML Protein, promyelocytic leukemia Protein, PML Protein, Pml Protein
- Background
- The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
- Molecular Weight
- 61.8 kDa
- NCBI Accession
- NP_150247
- Pathways
- p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
-
