Phosphomannomutase 2 (PMM2) protein (His tag)

Details for Product No. ABIN2729214, Supplier: Log in to see
Protein Name
  • cb626
  • zgc:56149
  • MGC82869
  • pmm2
  • CDG1
  • CDG1a
  • CDGS
  • PMI
  • PMI1
  • PMM 2
  • AI585868
  • C86848
  • phosphomannomutase 2
  • phosphomannomutase 2 S homeolog
  • pmm2
  • PMM2
  • pmm2.S
  • PTRG_02815
  • Tsp_00480
  • Pmm2
Origin
Human
11
1
1
1
Source
Escherichia coli (E. coli)
6
2
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This PMM2 protein is labelled with His tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human PMM2 protein expressed in E. coli.
  • Produced with end-sequenced ORF clone
Purity > 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin Level < 0.1 EU per μg protein as determined by LAL test
Protein Name
Background The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I.
Molecular Weight 29.1 kDa
NCBI Accession NP_000294
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 20 mM Tris-HCl, 150 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for Phosphomannomutase 2 (PMM2) protein (His tag) (ABIN2729214) Validation with Western Blot
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