CPI-17 Protein (His tag)
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- Target See all CPI-17 (PPP1R14A) Proteins
- CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CPI-17 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PPP1R14A protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product PPP1R14A Protein
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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) (AA 1-147) protein (GST tag)
PPP1R14A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) (AA 1-147) protein (Strep Tag)Crystallography grade PPP1R14A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) (AA 1-147) protein (Strep Tag)Crystallography grade PPP1R14A Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A) protein (Myc-DYKDDDDK Tag)PPP1R14A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 20 mM Tris-HCl, 0.2 mM EDTA, 1 mM DTT, 10 % Glycerol, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))
- Alternative Name
- Ppp1r14a (PPP1R14A Products)
- Synonyms
- MGC81874 Protein, cpi-17 Protein, ppp1inl Protein, CPI-17 Protein, im:7147597 Protein, ppp1r14a Protein, zgc:110700 Protein, PPP1R14A Protein, CPI17 Protein, PPP1INL Protein, Cpi17 Protein, 1110001M11Rik Protein, protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog Protein, protein phosphatase 1 regulatory inhibitor subunit 14A Protein, protein phosphatase 1, regulatory (inhibitor) subunit 14Aa Protein, protein phosphatase 1, regulatory (inhibitor) subunit 14A Protein, ppp1r14a.L Protein, ppp1r14a Protein, ppp1r14aa Protein, PPP1R14A Protein, Ppp1r14a Protein
- Background
- Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 17.8 kDa
- NCBI Accession
- NP_150281
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