SMAD1 Protein (His tag)

Catalog No. ABIN2732216

Product Details

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SMAD1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SMAD1 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: Smad1 (SMAD1 Products)

Synonyms: MLP Protein, Xmad1 Protein, Xsmad1 Protein, bsp1 Protein, jv4-1 Protein, jv41 Protein, madh1 Protein, madr1 Protein, smad-1 Protein, MADH1 Protein, fb39h09 Protein, wu:fb39h09 Protein, wu:fb97e04 Protein, 2/23 Protein, CG12399 Protein, Dmel\\CG12399 Protein, E(zen)2 Protein, En(vvl) Protein, MAD Protein, Mat Protein, P-Mad Protein, P-mad Protein, PMad Protein, Smad Protein, Smad1 Protein, apg Protein, c28 Protein, dMAD Protein, dMad Protein, l(2)K00237 Protein, l(2)k00237 Protein, mad Protein, p-Mad Protein, pMAD Protein, pMad Protein, pSmad Protein, BSP-1 Protein, BSP1 Protein, JV4-1 Protein, JV41 Protein, MADR1 Protein, AI528653 Protein, Madh1 Protein, Madr1 Protein, Mlp1 Protein, MusMLP Protein, smad1 Protein, xmad Protein, SMAD family member 1 Protein, Mothers against dpp Protein, SMAD family member 1 L homeolog Protein, smad1 Protein, SMAD1 Protein, Mad Protein, Smad1 Protein, smad1.L Protein

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Molecular Weight: 52.1 kDa

NCBI Accession: NP_005891

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development