SPC25 Protein (His tag)
-
- Target See all SPC25 Proteins
- SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SPC25 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human SPC25 / SPBC25 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SPC25 Protein
-
SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-224) protein (GST tag)
SPC25 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-224) protein (Strep Tag)Crystallography grade SPC25 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25) (AA 1-226) protein (Strep Tag)Crystallography grade SPC25 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the N-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- SPC25 (SPC25, NDC80 Kinetochore Complex Component, Homolog (SPC25))
- Alternative Name
- Spc25,spbc25 (SPC25 Products)
- Synonyms
- SPBC25 Protein, hSpc25 Protein, RGD1307811 Protein, Spbc25 Protein, spbc25 Protein, 2600017H08Rik Protein, 2610205L13Rik Protein, SPC25, NDC80 kinetochore complex component Protein, SPC25, NDC80 kinetochore complex component L homeolog Protein, SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) Protein, SPC25 Protein, Spc25 Protein, spc25.L Protein
- Background
- This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
- Molecular Weight
- 26.2 kDa
- NCBI Accession
- NP_065726
-
