STRADA Protein (His tag)

Catalog No. ABIN2732900

Product Details

Target: STRADA (STE20-Related Kinase Adaptor alpha (STRADA))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This STRADA protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human STRAD alpha / LYK5 (full length, N-term HIS tag, transcript variant 3) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: STRADA (STE20-Related Kinase Adaptor alpha (STRADA))

Alternative Name: Strad Alpha,lyk5 (STRADA Products)

Synonyms: fj99g05 Protein, zgc:56109 Protein, wu:fj99g05 Protein, lyk5 Protein, LYK5 Protein, NY-BR-96 Protein, PMSE Protein, STRAD Protein, Stlk Protein, Lyk5 Protein, 2610019A05Rik Protein, 6030402H20Rik Protein, AI480680 Protein, E130112C09Rik Protein, STE20-related kinase adaptor alpha Protein, ste20-related kinase adaptor alpha Protein, STRADA Protein, strada Protein, Strada Protein

Background: The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known.

Molecular Weight: 38.4 kDa

NCBI Accession: NP_699166

Pathways: AMPK Signaling