TYMS Protein (His tag)

Catalog No. ABIN2733655

Product Details

Target: TYMS (Thymidylate Synthetase (TYMS))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This TYMS protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Thymidylate synthase (TS) (full length, N-term polyhistidine tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase (Ts) (TYMS Products)

Synonyms: FN1 Protein, ECK2823 Protein, JW2795 Protein, ts Protein, Ts Protein, HST422 Protein, TMS Protein, TS Protein, thymidylate synthetase Protein, contains intron in T4 phage Protein, ThyE Protein, thymidylate synthase Protein, thymidylate synthetase L homeolog Protein, TYMS Protein, thyA Protein, td Protein, thyE Protein, DDA3937_RS04960 Protein, tyms.L Protein, XBJ1_RS15770 Protein, EAMY_RS20680 Protein, Tyms Protein

Target Type: Viral Protein

Background: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.

Molecular Weight: 36 kDa

NCBI Accession: NP_001062

Pathways: Mitotic G1-G1/S Phases