TIGAR Protein (TAT tag)
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- Target See all TIGAR Proteins
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TIGAR protein is labelled with TAT tag.
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human TIGAR protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 ug/mL, protects U2OS cells from apoptosis induced by hydrogen peroxide.
- Top Product
- Discover our top product TIGAR Protein
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TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 2-270) protein (His tag)
TIGAR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (GST tag)TIGAR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (Strep Tag)Crystallography grade TIGAR Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-269) protein (Strep Tag)Crystallography grade TIGAR Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
TP53 induced glycolysis regulatory phosphatase (TIGAR) protein (Myc-DYKDDDDK Tag)TIGAR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
- Alternative Name
- Tigar (TIGAR Products)
- Synonyms
- AA793651 Protein, AI595337 Protein, C79710 Protein, C85509 Protein, Tigar Protein, Trp53 induced glycolysis repulatory phosphatase Protein, Tigar Protein
- Background
- This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
- Molecular Weight
- 31.6 kDa
- NCBI Accession
- NP_065108
- Pathways
- Warburg Effect
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