NFE4 Protein (Myc-DYKDDDDK Tag)
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- Target See all NFE4 products
- NFE4 (Transcription Factor NF-E4 (NFE4))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NFE4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Transcription factor NF-E4 (NF-E4) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NFE4 (Transcription Factor NF-E4 (NFE4))
- Alternative Name
- Transcription Factor Nf-e4 (Nf-e4) (NFE4 Products)
- Synonyms
- NF-E4 Protein, nuclear factor, erythroid 4 Protein, NFE4 Protein
- Background
- The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand.
- Molecular Weight
- 18.9 kDa
- NCBI Accession
- NP_001078855
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