Thymic Stromal Lymphopoietin Protein (TSLP) (Transcript Variant 1)

Catalog No. ABIN2734447

This Recombinant Thymic Stromal Lymphopoietin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (Transcript Variant 1) (ABIN2734447)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: Transcript Variant 1

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human TSLP (transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 10^6 units/mg, determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 20 mM NaHPO4, pH 7.2, 0.15 M NaCl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: Tslp

Background: This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants.

Molecular Weight: 15 kDa

NCBI Accession: NP_149024