Nuclear Factor kappa B p50 (NFkB p50) (Active) protein (His tag)
Origin: Human
Host: Insect cells (Sf21)
Recombinant
>95 % (SDS-PAGE)
SDS
Active
-
- Target
- Nuclear Factor kappa B p50 (NFkB p50)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
-
Source
- Insect cells (Sf21)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human NF-kappaB (p50) is fused to a His-tag.
- Purity
- >95 % (SDS-PAGE)
-
Nuclear Factor kappa B p50 (NFkB p50) (full length ) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant GS, ELISA
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
~1ng is required for high mobility shift assay.~5ng are required for reconstituted transcription assays.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50-100 mM imidazole and 10 % glycerol.
- Storage
- -20 °C,-80 °C
- Storage Comment
-
Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - Expiry Date
- 6 months
-
- Target
- Nuclear Factor kappa B p50 (NFkB p50)
- Alternative Name
- NF-kB p50
- Synonyms
- NF-KB1 Protein, NF-kappaB Protein, NF-kappaB1 Protein, p105 Protein, p50 Protein, p50/p105 Protein, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Protein, Nfkb1 Protein
- Background
- NF-kappaB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappaB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at kappaB sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappaB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappaB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappaB inhibitor (I-kappaB) family. In a conventional activation pathway, I-kappaB is phosphorylated by I-kappaB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappaB complex which translocates to the nucleus. NF-kappaB heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappaB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3.
- UniProt
- P19838
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