KAT5 Protein (His tag)

Catalog No. ABIN2745869

Product Details

Target: KAT5 (K(lysine) Acetyltransferase 5 (KAT5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect cells (Sf21)

Purification tag / Conjugate: This KAT5 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Cross-Reactivity: Human

Characteristics: Human TIP60 is fused to a His-tag.

Purity: >95 % (SDS-PAGE)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

100-200ng are required for standard HAT assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2 % NP-40, 50 mM imidazole and 10 % glycerol.

Storage: -20 °C,-80 °C

Storage Comment: Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: KAT5 (K(lysine) Acetyltransferase 5 (KAT5))

Alternative Name: TIP60 (KAT5 Products)

Synonyms: 6121 Protein, CG6121 Protein, DmEG0007.7 Protein, Dmel/TIP60 Protein, Dmel\\CG6121 Protein, Dmel\\TIP60 Protein, EG0007.7 Protein, EG:EG0007.7 Protein, TIP60 Protein, dTIP60 Protein, dTip60 Protein, dmHAG405 Protein, im:6908768 Protein, wu:fb55b08 Protein, zgc:110752 Protein, ESA1 Protein, HTATIP Protein, HTATIP1 Protein, PLIP Protein, TIP Protein, ZC2HC5 Protein, cPLA2 Protein, AI839539 Protein, CPLA2 Protein, Htatip Protein, Htatip1 Protein, Tip55 Protein, Tip60 Protein, K(lysine) acetyltransferase 5a Protein, lysine acetyltransferase 5 Protein, Tat interactive protein 60kDa Protein, histone acetyltransferase Tip60 Protein, tip60, putative Protein, K(lysine) acetyltransferase 5b Protein, K(lysine) acetyltransferase 5 Protein, kat5a Protein, KAT5 Protein, Tip60 Protein, LOC413101 Protein, LOC5579231 Protein, Smp_053140 Protein, kat5b Protein, Kat5 Protein

Background: TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.

UniProt: Q92993

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway