TUBB Protein (AA 1-444) (Strep Tag)

Catalog No. ABIN3073868

Product Details

Target: TUBB (Tubulin, beta (TUBB))

Protein Type: Recombinant

Protein Characteristics: AA 1-444

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TUBB protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLDRISVY YNEATGGKYV PRAILVDLEP GTMDSVRSGP FGQIFRPDNF VFGQSGAGNN WAKGHYTEGA ELVDSVLDVV RKEAESCDCL QGFQLTHSLG GGTGSGMGTL LISKIREEYP DRIMNTFSVV PSPKVSDTVV EPYNATLSVH QLVENTDETY CIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQVFDAKNMM AACDPRHGRY LTVAAVFRGR MSMKEVDEQM LNVQNKNSSY FVEWIPNNVK TAVCDIPPRG LKMAVTFIGN STAIQELFKR ISEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEEDFGEEA EEEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB (TUBB Products)

Synonyms: M40 Protein, OK/SW-cl.56 Protein, TUBB1 Protein, TUBB5 Protein, XLOT Protein, m40 Protein, ok/sw-cl.56 Protein, tubb1 Protein, tubb5 Protein, TUBB2A Protein, TUBB2B Protein, TUBB Protein, 1t Protein, B1t Protein, BETA 56D Protein, CG9277 Protein, DTB2 Protein, Dmbeta1 Protein, Dmel\\CG9277 Protein, T Protein, Tub Protein, Tubulin Protein, beta Protein, beta-Tub Protein, beta-Tub56D Protein, beta-tub Protein, beta-tubulin56D Protein, beta1 Protein, beta1-Tubulin Protein, beta1-tub Protein, beta1Tub Protein, beta1t Protein, beta1tub Protein, beta56D Protein, betaTub Protein, betaTub1 Protein, beta[[1]] tubulin Protein, beta[[1]]-tubulin Protein, betatub(56D) Protein, 143391_i_at Protein, 3t Protein, B3t Protein, BETA 60D Protein, CG3401 Protein, D.m.BETA-60D Protein, DTB3 Protein, Dmbeta3 Protein, Dmel\\CG3401 Protein, Tub60D Protein, beta-Tub60D Protein, beta-Tub6D Protein, beta3 Protein, beta3 TU Protein, beta3-Tub Protein, beta3-tubulin Protein, beta3TUB Protein, beta3Tub Protein, beta3t Protein, beta60C Protein, betaTub3 Protein, betaTub60C Protein, beta[[3]] tubulin Protein, beta[[3]]-Tub Protein, beta[[3]]-tubulin Protein, betatub60D Protein, p50 Protein, p50/tubulin Protein, p53 Protein, p53/tubulin Protein, 2t Protein, B2t Protein, BETA 85D Protein, BETA2 Protein, CG9359 Protein, D.m.BETA-85D Protein, DTB4 Protein, Dmbeta2 Protein, Dmel\\CG9359 Protein, beta(2)Tu Protein, beta(2)Tub Protein, beta-Tub85D Protein, beta-tub85D Protein, beta2 Protein, beta2-tub Protein, beta2t Protein, beta2tub Protein, beta85D Protein, betaTub2 Protein, beta[[2]]-tubulin Protein, ms(3)KK[D] Protein, 4t Protein, B4t Protein, BETA 98B Protein, CG4869 Protein, DTB1 Protein, Dmbeta4 Protein, Dmel\\CG4869 Protein, beta-Tub97EF Protein, beta4 Protein, beta4t Protein, beta97F Protein, betaTub4 Protein, betaTub98BC Protein, betaTub98C Protein, Tub1 Protein, bmtub1 Protein, Tub3 Protein, bmtub3 Protein, Tub2 Protein, bmtub2 Protein, Tub4 Protein, bmtub4 Protein, LOC100101153 Protein, tubulin beta class I Protein, putative cobalt-zinc-cadmium outer membrane resistance protein Protein, Tubulin beta chain Protein, tubulin beta-2A chain Protein, beta-Tubulin at 56D Protein, beta-Tubulin at 60D Protein, beta-Tubulin at 85D Protein, beta-Tubulin at 97EF Protein, beta-tubulin Protein, tubulin beta 6 class V Protein, beta tubulin Protein, tubulin beta class I L homeolog Protein, tubulin, beta 5 class I Protein, TUBB Protein, czcC Protein, tbb-6 Protein, tubb Protein, LOC462399 Protein, betaTub56D Protein, betaTub60D Protein, betaTub85D Protein, betaTub97EF Protein, Tub1 Protein, Tub3 Protein, Tub2 Protein, Tub4 Protein, TVAG_525430 Protein, TVAG_062880 Protein, TVAG_456920 Protein, LOC100101153 Protein, TUBB6 Protein, Tb927.1.2330 Protein, Tb927.1.2350 Protein, Tb927.1.2370 Protein, Tb927.1.2390 Protein, tubb.L Protein, Tubb Protein, LOC693049 Protein, LOC443015 Protein, Tubb5 Protein

Background: Tubulin beta chain (Tubulin beta-5 chain),FUNCTION: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Molecular Weight: 49.7 kDa

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase