ZIC5 Protein (AA 1-663) (His tag)
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- Target See all ZIC5 products
- ZIC5 (Zinc Family Member 5 (ZIC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-663
- Origin
- Human
- Source
- Insect Cells
- Purification tag / Conjugate
- This ZIC5 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MFLKAGRGNK VPPVRVYGPD CVVLMEPPLS KRNPPALRLA DLATAQVQPL QNMTGFPALA GPPAHSQLRA AVAHLRLRDL GADPGVATTP LGPEHMAQAS TLGLSPPSQA FPAHPEAPAA AARAAALVAH PGAGSYPCGG GSSGAQPSAP PPPAPPLPPT PSPPPPPPPP PPPALSGYTT TNSGGGGSSG KGHSRDFVLR RDLSATAPAA AMHGAPLGGE QRSGTGSPQH PAPPPHSAGM FISASGTYAG PDGSGGPALF PALHDTPGAP GGHPHPLNGQ MRLGLAAAAA AAAAELYGRA EPPFAPRSGD AHYGAVAAAA AAALHGYGAV NLNLNLAAAA AAAAAGPGPH LQHHAPPPAP PPPPAPAQHP HQHHPHLPGA AGAFLRYMRQ PIKQELICKW IDPDELAGLP PPPPPPPPPP PPPPAGGAKP CSKTFGTMHE LVNHVTVEHV GGPEQSSHVC FWEDCPREGK PFKAKYKLIN HIRVHTGEKP FPCPFPGCGK VFARSENLKI HKRTHTGEKP FKCEFDGCDR KFANSSDRKK HSHVHTSDKP YYCKIRGCDK SYTHPSSLRK HMKIHCKSPP PSPGPLGYSS VGTPVGAPLS PVLDPARSHS STLSPQVTNL NEWYVCQASG APSHLHTPSS NGTTSETEDE EIYGNPEVVR TIH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ZIC5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ZIC5 (Zinc Family Member 5 (ZIC5))
- Alternative Name
- ZIC5 (ZIC5 Products)
- Synonyms
- XZic5 Protein, XlZic5 Protein, opl Protein, zic-1 Protein, zic1 Protein, zic5-A Protein, 1700049L20Rik Protein, Opr Protein, Zic family member 5 Protein, Zic family member 5 S homeolog Protein, zinc finger protein ZIC 5 Protein, zinc finger protein of the cerebellum 5 Protein, ZIC5 Protein, zic5.S Protein, Zic5 Protein, LOC787451 Protein
- Background
- Essential for neural crest development, converting cells from an epidermal fate to a neural crest cell fate. Binds to DNA (By similarity). {ECO:0000250}.
- Molecular Weight
- 69.4 kDa Including tag.
- UniProt
- Q96T25
- Pathways
- Tube Formation
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