ERAL1 Protein (AA 44-437) (His tag)
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- Target See all ERAL1 Proteins
- ERAL1 (Era G-Protein-Like 1 (ERAL1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 44-437
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This ERAL1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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VSCVAGSAFS GPRLASASRS NGQGSALDHF LGFSQPDSSV TPCVPAVSMN RDEQDVLLVH HPDMPENSRV LRVVLLGAPN AGKSTLSNQL LGRKVFPVSR KVHTTRCQAL GVITEKETQV ILLDTPGIIS PGKQKRHHLE LSLLEDPWKS MESADLVVVL VDVSDKWTRN QLSPQLLRCL TKYSQIPSVL VMNKVDCLKQ KSVLLELTAA LTEGVVNGKK LKMRQAFHSH PGTHCPSPAV KDPNTQSVGN PQRIGWPHFK EIFMLSALSQ EDVKTLKQYL LTQAQPGPWE YHSAVLTSQT PEEICANIIR EKLLEHLPQE VPYNVQQKTA VWEEGPGGEL VIQQKLLVPK ESYVKLLIGP KGHVISQIAQ EAGHDLMDIF LCDVDIRLSV KLLK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ERAL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ERAL1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ERAL1 (Era G-Protein-Like 1 (ERAL1))
- Alternative Name
- ERAL1 (ERAL1 Products)
- Synonyms
- ERAL1 Protein, 2610524P08Rik Protein, 9130407C09Rik Protein, AU019798 Protein, Era Protein, M-ERA Protein, MERA-S Protein, MERA-W Protein, GdERA Protein, si:ch211-207c6.1 Protein, ERA Protein, ERAL1A Protein, H-ERA Protein, HERA-A Protein, HERA-B Protein, eral1 Protein, Era like 12S mitochondrial rRNA chaperone 1 Protein, Era (G-protein)-like 1 (E. coli) Protein, Era-like 12S mitochondrial rRNA chaperone 1 Protein, Era-like 12S mitochondrial rRNA chaperone 1 L homeolog Protein, GTP-binding protein era homolog Protein, ERAL1 Protein, Eral1 Protein, eral1 Protein, eral1.L Protein, eral Protein
- Background
- Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly. {ECO:0000269|PubMed:20430825, ECO:0000269|PubMed:20604745}.
- Molecular Weight
- 44.4 kDa Including tag.
- UniProt
- O75616
- Pathways
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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