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GLYCTK Protein (AA 1-523) (Strep Tag)

GLYCTK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS
Catalog No. ABIN3080606
  • Target See all GLYCTK Proteins
    GLYCTK (Glycerate Kinase (GLYCTK))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-523
    Origin
    • 3
    • 1
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This GLYCTK protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MAAALQVLPR LARAPLHPLL WRGSVARLAS SMALAEQARQ LFESAVGAVL PGPMLHRALS LDPGGRQLKV RDRNFQLRQN LYLVGFGKAV LGMAAAAEEL LGQHLVQGVI SVPKGIRAAM ERAGKQEMLL KPHSRVQVFE GAEDNLPDRD ALRAALAIQQ LAEGLTADDL LLVLISGGGS ALLPAPIPPV TLEEKQTLTR LLAARGATIQ ELNTIRKALS QLKGGGLAQA AYPAQVVSLI LSDVVGDPVE VIASGPTVAS SHNVQDCLHI LNRYGLRAAL PRSVKTVLSR ADSDPHGPHT CGHVLNVIIG SNVLALAEAQ RQAEALGYQA VVLSAAMQGD VKSMAQFYGL LAHVARTRLT PSMAGASVEE DAQLHELAAE LQIPDLQLEE ALETMAWGRG PVCLLAGGEP TVQLQGSGRG GRNQELALRV GAELRRWPLG PIDVLFLSGG TDGQDGPTEA AGAWVTPELA SQAAAEGLDI ATFLAHNDSH TFFCCLQGGA HLLHTGMTGT NVMDTHLLFL RPR
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product GLYCTK Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    GLYCTK (Glycerate Kinase (GLYCTK))
    Alternative Name
    GLYCTK (GLYCTK Products)
    Synonyms
    HBEBP2 Protein, HBEBP4 Protein, HBeAgBP4A Protein, 6230410P16Rik Protein, C430006L12 Protein, zgc:153346 Protein, glycerate kinase Protein, GLYCTK Protein, Ndas_0956 Protein, Olsu_1363 Protein, Toce_0646 Protein, Acear_0799 Protein, Isop_1441 Protein, Sgly_3089 Protein, Odosp_0468 Protein, Bacsa_1428 Protein, Celly_0700 Protein, Deipr_2522 Protein, Corgl_1343 Protein, Spico_1775 Protein, Selsp_0613 Protein, Thena_0347 Protein, Halhy_0386 Protein, Mahau_0401 Protein, Glyctk Protein, glyctk Protein
    Background
    Glycerate kinase (EC 2.7.1.31) (HBeAg-binding protein 4)
    Molecular Weight
    55.3 kDa
    UniProt
    Q8IVS8
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