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LRRIQ3 Protein (AA 1-624) (Strep Tag)

LRRIQ3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS
Catalog No. ABIN3083021
  • Target See all LRRIQ3 products
    LRRIQ3 (Leucine-Rich Repeats and IQ Motif Containing 3 (LRRIQ3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-624
    Origin
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This LRRIQ3 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MFHGTVTEEL TSHEEWSHYN ENIREGQKDF VFVKFNGLHL KSMENLQSCI SLRVCIFSNN FITDIHPLQS CIKLIKLDLH GNQIKSLPNT KFWNGLKNLK LLYLHDNGFA KLKNICVLSA CPTLIALTMF DCPVSLKKGY RHVLVNSIWP LKALDHHVIS DEEIIQNWHL PERFKACNHR LFFNFCPALR KGTTYEEEIN NIKHITSKIN AILAHNSPVL IVQRWIRGFL VRKNLSPVFF HKKKQQEKII RGYEAKWIYI TKGYEDKLLK DLFFKPETNI KGKLAYWKHN IYYPVDLKNS SEHRKHVSSI LCELKPKDLG MKSKTSRHLI QKGQESEDEI VDEKLDTSFR ISVFKLPIYT SGSLKNNAVL REKKQHFFPA YPQPIYTTHP KPIIKKDIRL ERSMKEFFAP QRAGMKLRTF SDIDKYYTEQ KKQEYHKEKV RVVAMAQVAR ERVRVAVNEH LNQKKYATQK LIEENKETIQ NSLRQVWQNR FNYLEKARER KALFLKEKSQ KASERLLVQN LNNERTLLTR GLLKIDRLEK NEAVLKEKSL IVKQKLKAEK YRKNLLKEMK KVRSQEIYKR HCEEKFVMDM IAFEKACERL QDAKTKVAIV KTNLDFKVPN GLIK
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    LRRIQ3 (Leucine-Rich Repeats and IQ Motif Containing 3 (LRRIQ3))
    Alternative Name
    LRRIQ3 (LRRIQ3 Products)
    Synonyms
    LRRC44 Protein, 4930438B07Rik Protein, 4930511J15Rik Protein, 4933403H06Rik Protein, BB020367 Protein, Lrrc44 Protein, leucine rich repeats and IQ motif containing 3 Protein, leucine-rich repeats and IQ motif containing 3 Protein, LRRIQ3 Protein, Lrriq3 Protein
    Background
    Leucine-rich repeat and IQ domain-containing protein 3 (Leucine-rich repeat-containing protein 44)
    Molecular Weight
    73.7 kDa
    UniProt
    A6PVS8
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