ATG7 Protein (AA 2-703) (His tag)

Catalog No. ABIN3089598

Product Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Protein Type: Recombinant

Protein Characteristics: AA 2-703

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ATG7 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: AAATGDPGLS KLQFAPFSSA LDVGFWHELT QKKLNEYRLD EAPKDIKGYY YNGDSAGLPA RLTLEFSAFD MSAPTPARCC PAIGTLYNTN TLESFKTADK KLLLEQAANE IWESIKSGTA LENPVLLNKF LLLTFADLKK YHFYYWFCYP ALCLPESLPL IQGPVGLDQR FSLKQIEALE CAYDNLCQTE GVTALPYFLI KYDENMVLVS LLKHYSDFFQ GQRTKITIGV YDPCNLAQYP GWPLRNFLVL AAHRWSSSFQ SVEVVCFRDR TMQGARDVAH SIIFEVKLPE MAFSPDCPKA VGWEKNQKGG MGPRMVNLSE CMDPKRLAES SVDLNLKLMC WRLVPTLDLD KVVSVKCLLL GAGTLGCNVA RTLMGWGVRH ITFVDNAKIS YSNPVRQPLY EFEDCLGGGK PKALAAADRL QKIFPGVNAR GFNMSIPMPG HPVNFSSVTL EQARRDVEQL EQLIESHDVV FLLMDTRESR WLPAVIAASK RKLVINAALG FDTFVVMRHG LKKPKQQGAG DLCPNHPVAS ADLLGSSLFA NIPGYKLGCY FCNDVVAPGD STRDRTLDQQ CTVSRPGLAV IAGALAVELM VSVLQHPEGG YAIASSSDDR MNEPPTSLGL VPHQIRGFLS RFDNVLPVSL AFDKCTACSS KVLDQYEREG FNFLAKVFNS SHSFLEDLTG LTLLHQETQA AEIWDMSDDE TI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ATG7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Synonyms: APG7-LIKE Protein, APG7L Protein, GSA7 Protein, D8 Protein, UBA1B Protein, UBE1L Protein, UBE2 Protein, Ube1l Protein, 1300004C08Rik Protein, AO090001000744 Protein, atg7 Protein, 1810013K23Rik Protein, Agp7 Protein, Apg7l Protein, Atg7l Protein, ATAPG7 Protein, ATATG7 Protein, ATG7 Protein, AUTOPHAGY 7 Protein, AUTOPHAGY-RELATED 7 Protein, K15I22.10 Protein, K15I22_10 Protein, autophagy related 7 Protein, ubiquitin like modifier activating enzyme 7 Protein, ubiquitin-like modifier activating enzyme 7 Protein, autophagy-related protein 7 Protein, Autophagy-related protein 7 Protein, Apg7p Protein, ThiF family protein Protein, ubiquitin-like conjugating enzyme Atg7 Protein, ATG7 Protein, UBA7 Protein, Uba7 Protein, ANI_1_3368024 Protein, AOR_1_1338164 Protein, PTRG_02236 Protein, PAAG_00287 Protein, PITG_12537 Protein, atg7 Protein, Atg7 Protein, APG7 Protein

Background: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. {ECO:0000269|PubMed:11096062, ECO:0000269|PubMed:16303767, ECO:0000269|PubMed:22170151}.

Molecular Weight: 78.8 kDa Including tag.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy