CCDC61 Protein (AA 1-512) (Strep Tag)
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- Target See all CCDC61 products
- CCDC61 (Coiled-Coil Domain Containing 61 (CCDC61))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-512
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Origin
- Human
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Source
- Tobacco (Nicotiana tabacum)
- Purification tag / Conjugate
- This CCDC61 protein is labelled with Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MDQPAGLQVD YVFRGVEHAV RVMVSGQVLE LEVEDRMTAD QWRGEFDAGF IEDLTHKTGN FKQFNIFCHM LESALTQSSE SVTLDLLTYT DLESLRNRKM GGRPGSLAPR SAQLNSKRYL ILIYSVEFDR IHYPLPLPYQ GKPDPVVLQG IIRSLKEELG RLQGLDGQNT RDTRENEIWH LREQVSRLAS EKRELEAQLG RSREEALAGR AARQEAEALR GLVRGLELEL RQERGLGHRV AGRRGQDCRR LAKELEEAKA SERSLRARLK TLTSELALYK RGRRTPPVQP PPTREDRASS SRERSASRGR GAARSSSRES GRGSRGRGRP ARPSPSPTGG RALRFDPTAF VKAKERKQRE IQMKQQQRNR LGSGGSGDGP SVSWSRQTQP PAALTGRGDA PNRSRNRSSS VDSFRSRCSS ASSCSDLEDF SESLSRGGHR RRGKPPSPTP WSGSNMKSPP VERSHHQKSL ANSGGWVPIK EYSSEHQAAD MAEIDARLKA LQEYMNRLDM RS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Purity
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CCDC61 (Coiled-Coil Domain Containing 61 (CCDC61))
- Alternative Name
- CCDC61 (CCDC61 Products)
- Synonyms
- C530028I08Rik Protein, Gm159 Protein, RGD1307390 Protein, coiled-coil domain containing 61 Protein, CCDC61 Protein, Ccdc61 Protein
- Background
- Centrosomal protein CCDC61 (Coiled-coil domain-containing protein 61) (VFL3 homolog),FUNCTION: Microtubule-binding centrosomal protein required for centriole cohesion, independently of the centrosome-associated protein/CEP250 and rootletin/CROCC linker (PubMed:31789463). In interphase, required for anchoring microtubule at the mother centriole subdistal appendages and for centrosome positioning (PubMed:31789463). During mitosis, may be involved in spindle assembly and chromatin alignment by regulating the organization of spindle microtubules into a symmetrical structure (PubMed:30354798). Has been proposed to play a role in CEP170 recruitment to centrosomes (PubMed:30354798). However, this function could not be confirmed (PubMed:31789463). Plays a non-essential role in ciliogenesis (PubMed:31789463, PubMed:32375023). {ECO:0000269|PubMed:30354798, ECO:0000269|PubMed:31789463, ECO:0000269|PubMed:32375023}.
- Molecular Weight
- 57.4 kDa
- UniProt
- Q9Y6R9
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