CFTR Protein (AA 1152-1480) (His tag)
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- Target See all CFTR Proteins
- CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1152-1480
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This CFTR protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
- MDVDSLMRSV SRVFKFIDMP TEGKPTKSTK PYKNGQLSKV MIIENSHVKK DDIWPSGGQM TVKDLTAKYT EGGNAILENI SFSISPGQRV GLLGRTGSGK STLLSAFLRL LNTEGEIQID GVSWDSITLQ QWRKAFGVIP QKVFIFSGTF RKNLDPYEQW SDQEIWKVAD EVGLRSVIEQ FPGKLDFVLV DGGCVLSHGH KQLMCLARSV LSKAKILLLD EPSAHLDPVT YQIIRRTLKQ AFADCTVILC EHRIEAMLEC QQFLVIEENK VRQYDSIQKL LNERSLFRQA ISPSDRVKLF PHRNSSKCKS KPQIAALKEE TEEEVQDTRL HHHHHH
- Characteristics
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This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 1381-1480) protein (GST tag)
CFTR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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C-terminal His-tag
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Hepes, pH7.4; 100 mM NaCl
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))
- Alternative Name
- CFTR (CFTR Products)
- Synonyms
- ABC35 Protein, ABCC7 Protein, CF Protein, CFTR/MRP Protein, MRP7 Protein, TNR-CFTR Protein, dJ760C5.1 Protein, CFTR Protein, AW495489 Protein, Abcc7 Protein, RGD1561193 Protein, abc35 Protein, abcc7 Protein, cftr/mrp Protein, mrp7 Protein, tnr-cftr Protein, xcftr Protein, si:dkey-270i2.2 Protein, cystic fibrosis transmembrane conductance regulator Protein, cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) Protein, CFTR Protein, cftr-A Protein, Cftr Protein, cftr Protein
- Background
- Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. {ECO:0000269|PubMed:22178883}.
- Molecular Weight
- 38.4 kDa Including tag.
- UniProt
- P13569
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