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CEP97 Protein (AA 1-865) (Strep Tag)

CEP97 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, WB, SDS
Catalog No. ABIN3091511
  • Target See all CEP97 Proteins
    CEP97 (Centrosomal Protein 97kDa (CEP97))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-865
    Origin
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This CEP97 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MAVARVDAAL PPGEGSVVNW SGQGLQKLGP NLPCEADIHT LILDKNQIIK LENLEKCKRL IQLSVANNRL VRMMGVAKLT LLRVLNLPHN SIGCVEGLKE LVHLEWLNLA GNNLKAMEQI NSCTALQHLD LSDNNISQIG DLSKLVSLKT LLLHGNIITS LRMAPAYLPR SLAILSLAEN EIRDLNEISF LASLTELEQL SIMNNPCVMA TPSIPGFDYR PYIVSWCLNL RVLDGYVISQ KESLKAEWLY SQGKGRAYRP GQHIQLVQYL ATVCPLTSTL GLQTAEDAKL EKILSKQRFH QRQLMNQSQN EELSPLVPVE TRASLIPEHS SPVQDCQISQ ESEPVIQVNS WVGINSNDDQ LFAVKNNFPA SVHTTRYSRN DLHLEDIQTD EDKLNCSLLS SESTFMPVAS GLSPLSPTVE LRLQGINLGL EDDGVADESV KGLESQVLDK EEEQPLWAAN ENSVQMMRSE INTEVNEKAG LLPCPEPTII SAILKDDNHS LTFFPESTEQ KQSDIKKPEN TQPENKETIS QATSEKLPMI LTQRSVALGQ DKVALQKLND AATKLQACWR GFYARNYNPQ AKDVRYEIRL RRMQEHIVCL TDEIRRLRKE RDEERIKKFV QEEAFRFLWN QVRSLQVWQQ TVDQRLSSWH TDVPPISSTL VPSKHPLFTQ SQESSCDQNA DWFIASDVAP QEKSLPEFPD SGFHSSLTEQ VHSLQHSLDF EKSSTEGSES SIMGNSIDTV RYGKESDLGD VSEEHGEWNK ESSNNEQDNS LLEQYLTSVQ QLEDADERTN FDTETRDSKL HIACFPVQLD TLSDGASVDE SHGISPPLQG EISQTQENSK LNAEVQGQQP ECDSTFQLLH VGVTV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product CEP97 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CEP97 (Centrosomal Protein 97kDa (CEP97))
    Alternative Name
    CEP97 (CEP97 Products)
    Synonyms
    MGC83642 Protein, 2810403B08Rik Protein, 4932439K18Rik Protein, AA437768 Protein, E130116N02Rik Protein, Lrriq2 Protein, RGD1307400 Protein, LRRIQ2 Protein, centrosomal protein 97kDa L homeolog Protein, centrosomal protein 97 Protein, centrosomal protein of 97 kDa Protein, cep97.L Protein, CEP97 Protein, LOC100459112 Protein, cep97 Protein, Cep97 Protein
    Background
    Centrosomal protein of 97 kDa (Cep97) (Leucine-rich repeat and IQ domain-containing protein 2),FUNCTION: Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation (PubMed:17719545, PubMed:30375385). Required for recruitment of CCP110 to the centrosome (PubMed:17719545). {ECO:0000269|PubMed:17719545, ECO:0000269|PubMed:30375385}.
    Molecular Weight
    97.0 kDa
    UniProt
    Q8IW35
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