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DLGAP2 Protein (AA 1-1054) (Strep Tag)

DLGAP2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
Catalog No. ABIN3092110
  • Target See all DLGAP2 Proteins
    DLGAP2 (Discs, Large (Drosophila) Homolog-Associated Protein 2 (DLGAP2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1054
    Origin
    Human
    Source
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This DLGAP2 protein is labelled with Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MGTAQVLPGI LQKHCCILPD RNTESQCTLC GEPEEEEAGD LVQPGISFPG PAEEDLDPQY SWSPTQHFNE ERYSPAPRSM KGLSGSRTQP PLCSGHTCGL APPEDCEHLH HGPDARPPYL LSPADSCPGG RHRCSPRSSV HSECVMMPVV LGDHVSSSTF PRMHYSSHYD TRDDCAVAHA GAKINRIPAN LLDQFEKQLP LHRDGFHTLQ YQRTSAAAEQ RSESPGRIRH LVHSVQKLFT KSHSLEGSSK SNANGTKADG RADDHHHAHH AKHSKRSKSK ERKPEGKPRP GMSSWWSSDD NLDSDSTYRT PSVLNRHHLG PVAHCYPDAL QSPFGDLSLK TSKSNNDVKC SACEGLALTP DAKYLKRSSW STLTVSQAKE AYRKSSLNLD KPLLHQDAKP ALRPCHYLQV PQDEWGGYPT GGKDEEIPCR RMRSGSYIKA MGDEESGESD SSPKTSPKSA ILPEPLLKSI GQRPLGEHQT QTYLQAASDV PVGHSLDPAA NYNSPKFRSR NQSYMRAVST LSQASCVSQV SEAEINGQFE SVCESVFSEV ESQAMDALDL PGCFRTRSHS YLRAIQAGYS QDDECIPMMT PSDITSTIRS TAAVSYTNYK KTPPPVPPRT TSKPLISVTA QSSTESTQDA YQDSRAQRMS PWPQDSRGLY NSTDSLDSNK AMNLALETAA AQRHLPESQS SSVRTSDKAI LVSKAEELLK SRCSSIGIQD SEFPEHQPYP RSDVETATDS DTESRGLREY HSVGVQVEDE KRHGRFKRSN SVTAAVQADL ELEGFPGHIT TEDKGLQFGS SFQRHSEPST PTQYSAVRTV RTQGLFSYRE DYRTQVDTST LPPPDPWLEP AIDTVETGRM SPCRRDGSWF LKLLHAETKR MEGWCKEMER EAEENDLSEE ILGKIRSAVG SAQLLMSQKF QQFYWLCQQN MDPSAMPRPT SQDLAGYWDM LQLSIEDVSM KFDELQRLRL NDWKMMESPE RKEERKVPPP IPKKPPKGKF PITREKSLDL PDRQRQEARR RLMAAKRAAS FRQNSASERA DSIEIYIPEA QTRL
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    DLGAP2 (Discs, Large (Drosophila) Homolog-Associated Protein 2 (DLGAP2))
    Alternative Name
    DLGAP2 (DLGAP2 Products)
    Synonyms
    PSD-95 Protein, DAP2 Protein, SAPAP2 Protein, 6430596N04Rik Protein, Sapap2 Protein, DLG associated protein 2 Protein, disks large-associated protein 2 Protein, discs, large (Drosophila) homolog-associated protein 2a Protein, discs, large (Drosophila) homolog-associated protein 2 Protein, DLGAP2 Protein, LOC463960 Protein, dlgap2a Protein, LOC100447448 Protein, Dlgap2 Protein
    Background
    Disks large-associated protein 2 (DAP-2) (PSD-95/SAP90-binding protein 2) (SAP90/PSD-95-associated protein 2) (SAPAP2),FUNCTION: May play a role in the molecular organization of synapses and neuronal cell signaling. Could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. May induce enrichment of PSD-95/SAP90 at the plasma membrane.
    Molecular Weight
    117.6 kDa
    UniProt
    Q9P1A6
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